Journal: Nutrients

Article Title: Vitamin C Activates Osteoblastogenesis and Inhibits Osteoclastogenesis via Wnt/β-Catenin/ATF4 Signaling Pathways

doi: 10.3390/nu11030506

Figure Lengend Snippet: Expression of both osteoblast- and osteoclast-regulated proteins in vitamin C-treated rat tibias. ( A ) Western blot image of Wnt3a, β-catenin, and ATF4 and quantitative assay of Wnt3a, β-catenin, and ATF4 protein expression in vitamin C-treated rat tibias. ( B ) Western blot image of p-AKT, p-ERK, p-p38, and p-JNK and quantitative assay of p-AKT, p-ERK, p-p38, and p-JNK protein expression in vitamin C-treated rat tibias. Expression was quantified using ImageJ software relative to that of β-actin. Values represent the mean ± standard deviation. Values with different letters were significantly different according to Duncan’s multiple range test ( P < 0.05).

Article Snippet: Membranes were blocked with 5% bovine serum albumin prior to incubation with specific primary antibodies against BMP-2, RUNX2, Wnt3a, osteocalcin, COL-1 (Abcam, Cambridge, UK), SMAD1/5/8 (Santa Cruz Biotechnology, Dallas, TX, USA), ATF4 (Boster, Pleasanton, CA, USA), osteoprotegerin (OPG), RANK, RANKL (Bioss Antibodies, Woburn, MA, USA), TRAP, cathepsin K (GeneTex, Irvine, CA, USA), β-catenin, phosphorylated serine/threonine kinase (p-AKT), phosphorylated extracellular signal-regulated kinase (p-ERK), p-p38, phosphorylated c-Jun N-terminal kinase (p-JNK), and β-actin (Cell Signaling Technology, Danvers, MA, USA).

Techniques: Expressing, Western Blot, Software, Standard Deviation

Journal: Oncology reports

Article Title: Gonadotropins promote human ovarian cancer cell migration and invasion via a cyclooxygenase 2-dependent pathway.

doi: 10.3892/or.2017.5784

Figure Lengend Snippet: Figure 1. FSHR and LHR were expressed in SKOV3 and HO8910 cells but did not affect cell proliferation and apoptosis. Expression of FSHR and LHR was examined by immunocytochemical staining (A), RT-PCR (B), and western blotting (C). HO8910 and SKOV3 cells were treated with FSH and LH (0, 100, and 500 mIU/ml) alone or in combination, but no changes in proliferation (D and E) and apoptosis (F) were observed.

Article Snippet: Antibodies specific to FSHR (cat. PB1120; 1:200) and LHR (cat. A01120; 1:200) were obtained from Boster.

Techniques: Expressing, Staining, Reverse Transcription Polymerase Chain Reaction, Western Blot

Journal: Iranian Journal of Basic Medical Sciences

Article Title: The impact of moderate-intensity swimming exercise on learning and memory in aged rats: The role of Sirtuin-1

doi: 10.22038/IJBMS.2021.58145.12920

Figure Lengend Snippet: The time spent on the destination quadrant as a groups

Article Snippet: The sections were stained with Sirtuin-1 [Anti-SIRT-1 antibody [E104] (ab32441), Abcam, 1/100 dilution], BDNF [Anti-BDNF Picoband antibody (PB9075), BosterBio, 1/100 dilution] and CREB [Anti-CREB/CREB1 Picoband antibody (PB9100), BosterBio, 1/100 dilution] according to the immunohistochemical procedure to determine the reaction.

Techniques: Control

Journal: Iranian Journal of Basic Medical Sciences

Article Title: The impact of moderate-intensity swimming exercise on learning and memory in aged rats: The role of Sirtuin-1

doi: 10.22038/IJBMS.2021.58145.12920

Figure Lengend Snippet: CREB expressions in hippocampus between the groups

Article Snippet: The sections were stained with Sirtuin-1 [Anti-SIRT-1 antibody [E104] (ab32441), Abcam, 1/100 dilution], BDNF [Anti-BDNF Picoband antibody (PB9075), BosterBio, 1/100 dilution] and CREB [Anti-CREB/CREB1 Picoband antibody (PB9100), BosterBio, 1/100 dilution] according to the immunohistochemical procedure to determine the reaction.

Techniques:

Journal: Iranian Journal of Basic Medical Sciences

Article Title: The impact of moderate-intensity swimming exercise on learning and memory in aged rats: The role of Sirtuin-1

doi: 10.22038/IJBMS.2021.58145.12920

Figure Lengend Snippet: SIRT-1, BDNF and CREB statistical analysis of immunohistochemical positive cell numbers of hippocampus of rats between the groups

Article Snippet: The sections were stained with Sirtuin-1 [Anti-SIRT-1 antibody [E104] (ab32441), Abcam, 1/100 dilution], BDNF [Anti-BDNF Picoband antibody (PB9075), BosterBio, 1/100 dilution] and CREB [Anti-CREB/CREB1 Picoband antibody (PB9100), BosterBio, 1/100 dilution] according to the immunohistochemical procedure to determine the reaction.

Techniques: Immunohistochemical staining, Control

Journal: Frontiers in Immunology

Article Title: Granulocytic Myeloid-Derived Suppressor Cell Exosomal Prostaglandin E2 Ameliorates Collagen-Induced Arthritis by Enhancing IL-10 + B Cells

doi: 10.3389/fimmu.2020.588500

Figure Lengend Snippet: G-MDSC exosomal PGE2 promotes IL-10 + B cells (A) COX-2 expression in G-MDSCs was detected after treatment with the COX-2 inhibitor celecoxib. (B) The ratio of COX2 and GAPDH in each group was statistically analyzed. (C) The level of PGE2 in the G-MDSC culture supernatant treated with celecoxib was detected by ELISA. (D) The level of PGE2 in the G-exo treated with celecoxib was detected by ELISA. (E) IL-10–producing B cells after treatment with celecoxib-treated G-exo in the present of LPS were analyzed by flow cytometry. (F) The level of IL-10 in culture supernatant after treatment with celecoxib-treated G-exo was detected by ELISA. (G) The p-GSK-3β, T-GSK-3β, p-CREB, and T-CREB were detected by Western blot analysis. (H) The ratio of p-CREB and T-CREB in each group was statistically analyzed. (I) The ratio of p-GSK-3β and T-GSK-3β in each group was statistically analyzed. Bar graphs show the means ± SD. *P < 0.05; **P < 0.01; ***P < 0.001; ns: indicates no significance.

Article Snippet: The membranes were then incubated with specific rabbit antibodies against phosphorylated (p)-GSK-3β (Santa Cruz Biotechnology, Texas, USA ) , total (t)- GSK-3β (Wanleibio, Shenyang, China), phosphorylated (p)-cAMP response element binding protein (CREB) (Wanleibio, Shenyang, China) and total (t)-CREB (Wanleibio, Shenyang, China) followed by an incubation with the secondary HRP-conjugated goat anti-rabbit IgG (CST, Danvers, MA, USA) according to manufacturer’s protocol.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Western Blot

Journal: Frontiers in Immunology

Article Title: Granulocytic Myeloid-Derived Suppressor Cell Exosomal Prostaglandin E2 Ameliorates Collagen-Induced Arthritis by Enhancing IL-10 + B Cells

doi: 10.3389/fimmu.2020.588500

Figure Lengend Snippet: Schematic image demonstrating that Granulocytic myeloid-derived suppressor cell exosomal prostaglandin E2 ameliorates collagen-induced arthritis by inducing IL-10 + B cells production via affecting GSK-3β and CREB phosphorylation.

Article Snippet: The membranes were then incubated with specific rabbit antibodies against phosphorylated (p)-GSK-3β (Santa Cruz Biotechnology, Texas, USA ) , total (t)- GSK-3β (Wanleibio, Shenyang, China), phosphorylated (p)-cAMP response element binding protein (CREB) (Wanleibio, Shenyang, China) and total (t)-CREB (Wanleibio, Shenyang, China) followed by an incubation with the secondary HRP-conjugated goat anti-rabbit IgG (CST, Danvers, MA, USA) according to manufacturer’s protocol.

Techniques: Derivative Assay, Phospho-proteomics